Journal: Tropical Medicine and Infectious Disease

Article Title: Genetic Classification of a Novel Genotype of the Genus Acanthamoeba Isolated from Tap Water in Mexico

doi: 10.3390/tropicalmed11040093

Figure Lengend Snippet: Electrophoresis gel of Acanthamoeba positive samples. Lane 1: Hyper-Ladder 100 bp™ molecular weight marker; lanes 2–9: ASA.S1 region amplicons for positive samples.

Article Snippet: The mapping of the 18S rRNA gene was performed using the sequence of Acanthamoeba castellanii Ma ATCC 50370 18S rRNA, sourced from NCBI [ ], as a reference with Bowtie2 software v2.5.4 [ ].

Techniques: Electrophoresis, Molecular Weight, Marker

Journal: Tropical Medicine and Infectious Disease

Article Title: Genetic Classification of a Novel Genotype of the Genus Acanthamoeba Isolated from Tap Water in Mexico

doi: 10.3390/tropicalmed11040093

Figure Lengend Snippet: Morphological characterisation of the LUDO4 strain of Acanthamoeba . ( A ) General view of the population at 100× magnification, showing a heterogeneous distribution with large trophozoites and emerging cysts. ( B ) Trophozoites visualized at 1000× magnification; the cells shown represent the lower end of the size spectrum (≈33 μm) according to the morphometric analysis of 25 individuals.

Article Snippet: The mapping of the 18S rRNA gene was performed using the sequence of Acanthamoeba castellanii Ma ATCC 50370 18S rRNA, sourced from NCBI [ ], as a reference with Bowtie2 software v2.5.4 [ ].

Techniques:

Journal: Tropical Medicine and Infectious Disease

Article Title: Genetic Classification of a Novel Genotype of the Genus Acanthamoeba Isolated from Tap Water in Mexico

doi: 10.3390/tropicalmed11040093

Figure Lengend Snippet: Maximum Likelihood tree for reference sequences of Acanthamoeba genotypes from NCBI and ASA.S1 sequences isolated from tap water in Cajeme, Sonora, Mexico. Isolates from this study are highlighted in blue.

Article Snippet: The mapping of the 18S rRNA gene was performed using the sequence of Acanthamoeba castellanii Ma ATCC 50370 18S rRNA, sourced from NCBI [ ], as a reference with Bowtie2 software v2.5.4 [ ].

Techniques: Isolation

Journal: Tropical Medicine and Infectious Disease

Article Title: Genetic Classification of a Novel Genotype of the Genus Acanthamoeba Isolated from Tap Water in Mexico

doi: 10.3390/tropicalmed11040093

Figure Lengend Snippet: A maximum likelihood phylogenetic tree supporting the identification of a novel genotype T24. Tree based on reference 18S rRNA sequences of Acanthamoeba and the complete 18S rRNA region of strain LUDO1, showing the novel genotype positioned as a sister lineage to the T15/T22 clade. Isolates from this study are highlighted in blue.

Article Snippet: The mapping of the 18S rRNA gene was performed using the sequence of Acanthamoeba castellanii Ma ATCC 50370 18S rRNA, sourced from NCBI [ ], as a reference with Bowtie2 software v2.5.4 [ ].

Techniques:

Journal: Tropical Medicine and Infectious Disease

Article Title: Genetic Classification of a Novel Genotype of the Genus Acanthamoeba Isolated from Tap Water in Mexico

doi: 10.3390/tropicalmed11040093

Figure Lengend Snippet: Cyst morphology of Acanthamoeba genotype T24 at 1000× magnification. ( A , B ) Representative mature cysts showing the characteristic double-walled structure of Group III. ( B ) The ectocyst (outer wall) appears wrinkled or irregular, while the endocyst (inner wall) has a slightly more angular outline than the ectocyst.

Article Snippet: The mapping of the 18S rRNA gene was performed using the sequence of Acanthamoeba castellanii Ma ATCC 50370 18S rRNA, sourced from NCBI [ ], as a reference with Bowtie2 software v2.5.4 [ ].

Techniques:

Journal: Tropical Medicine and Infectious Disease

Article Title: Genetic Classification of a Novel Genotype of the Genus Acanthamoeba Isolated from Tap Water in Mexico

doi: 10.3390/tropicalmed11040093

Figure Lengend Snippet: A maximum likelihood phylogenetic tree supporting the identification of a novel genotype T24. Tree based on reference 18S rRNA sequences of Acanthamoeba and the complete 18S rRNA region of strain LUDO1, showing the novel genotype positioned as a sister lineage to the T15/T22 clade. Isolates from this study are highlighted in blue.

Article Snippet: The mapping of the 18S rRNA gene was performed using the sequence of Acanthamoeba castellanii Ma ATCC 50370 18S rRNA, sourced from NCBI [ ], as a reference with Bowtie2 software v2.5.4 [ ].

Techniques: